SciLicium Bioinformatics

SciLicium is based in Rennes (Brittany, France) and specialized in multi-omics data analysis and the development of workflows and web app solutions in bioinformatics. Over the last few years, SciLicium has also developed strong expertise in toxicogenomics (read-across, adverse outcome pathways, mechanisms of action) including predictive toxicogenomics based on statistical modeling (Machine and Deep Learning).

Determine the toxic potential of compounds is an important challenge faced by scientists and regulatory authorities alike (Grimm, 2011). The genomic-scale data rapid generation has led to the development of toxicogenomics. They combine classical toxicology approaches with (metabol-, prote-, transcript-, epigen-) omics technologies to identify not only deregulated molecular mechanisms upon exposures but also candidate biomarkers for toxicity prediction. In the risk assessment, process transcriptomics datasets (and their resulting gene expression signatures) are now used more and more to:

• (i) characterize the molecular mode of action of chemicals, small molecules, and nanomaterials exposure determined by their toxicogenomics signature (e.g. over/under-expressed genes after exposure to the compound) (Darde et al., 2018)
• (ii) predict the genomic behavior underlying the exposure and its toxicity (Liu et al., 2019)
• (iii) define the adverse outcome pathways (AOP) (Brockmeier et al., 2017)
• (iv) determine the benchmark dose and estimate the critical point of departure for human health risk assessment (Farmahin et al., 2017)
• (v) predict the behavior of uncharacterized compounds by comparing them to other substances whose molecular effects are known (read across) (Pawar et al., 2019)

In this context, SciLicium Toxicogenomics services aim at using transcriptomics data to address several challenges in toxicogenomics. After careful planning of exposure conditions, RNA extraction, and library preparation, the toxicogenomics data are pre-processed and used to identify the toxicogenomics signature, to perform functional analysis, and predict and prioritize potential adverse effects.

Services Overview

SciLicium Toxicogenomics services include:

• A strong expertise in toxicogenomics
• A know-how in toxicogenomics data analysis
• The development of data analysis workflows for standardizing procedures
• The deployment of a dedicated web application for sharing data and communication purposes.

Toxicogenomics expertise

Our experience in a wide range of sequencing technologies (DNA chips, RNA-seq, ChIP-seq, single-cell RNA-seq, 5 'or 3' RNA-seq) makes SciLicium the perfect partners for your projects.

• Experimental design

With its experience in a wide range of technologies, SciLicium can advise you on the optimal experimental and sequencing strategy to choose to acquire the toxicogenomics data set necessary for your project.

• Sequencing

Over the years, SciLicium has created an international collaborators network and makes it available to you for your sequencing project.

• Data management and analysis

SciLicium can get involved at different levels of your analysis: from guidance to help you define your data analysis and data management strategy to a more active role in analyzing your data and/or setting up pipelines and web tools.

Toxicogenomics data analysis

Basic, Standard, or Advanced, it is up to you to choose and customize the analysis that will suit your project. SciLicium’s experience in toxicogenomics data processing and analysis allows us to offer you various benefits on a wide range of technologies (DNA chips, RNA-seq, single-cell RNA-seq, 5 'or 3' RNA-seq) to screen your compounds.

Our Analyses

Workflows & web application development

Tailor-made workflows: standardization of your procedures
Automate your data analysis, save time, and increase your study reproducibility while being flexible enough to adapt to your project needs.
Through the various projects carried out by SciLicium, several analysis workflows (DGE-seq, BRB-seq, RNA-seq, single-cell RNA-seq, metagenomics) have been set up and adapted to answer your scientific questions. SciLicium uses the workflow editing standards (Snakemake, Nextflow, CWL) to offer analysis stability over time.

Deploy your bioinformatics tools online
Web application creation is now a major activity in the bioinformatics field in order to share research.
At SciLicium, we have acquired web development skills and expertise to deploy solutions. By working closely with you, we can help you develop innovative bioinformatics web applications with advanced and reliable technologies to meet your project-specific requirements.

SHOP

SciLicium offers a basic, standard, or advanced service for analyzing "-omics" data from different technologies (DNA chip, Single-cell RNA-seq, 3 'or 5' sequencing, metagenomics,. ..) fitting to the needs and scientific question.

See SciLicium Shop